اثرات ضد التهابی و محافظتی عصاره زعفران در آسیب حاد کلیوی ناشی از ایسکمی/پرفیوژن مجدد

التهاب یکی از چالش‌های اصلی در مدیریت آسیب حاد کلیوی ناشی از ایسکمی/پرفیوژن (I/R) است. هدف ارزیابی اثرات ضدالتهابی و محافظتی عصاره زعفران در برابر اختلالات کلیوی ناشی از I/R بود. در مطالعه‌ای بر روی ۳۵ موش ویستار، گروه‌هایی با دوزهای مختلف عصاره زعفران پیش‌تیمار شدند. پارامترهای التهاب (مانند نفوذ لکوسیت و بیان ژن TNF-α\alphaα) و استرس اکسیداتیو اندازه‌گیری شدند. نتایج نشان داد پیش‌درمان با عصاره زعفران به طور قابل توجهی شدت اختلالات را کاهش داد و اثرات ضدالتهابی، آنتی‌اکسیدانی و محافظتی عصاره هیدرو-اتانولی زعفران را در آسیب کلیوی ناشی از I/R اثبات کرد.

Inflammation is one of the major challenges in the management of ischaemia/reperfusion (I/R)-induced acute kidney injury. Our aim was to assess the anti-inflammatory and protective effects of saffron extract against I/R-induced renal disturbances. A total of 35 male Wistar rats were randomly divided into five groups (n = 7) as sham, I/R and three groups of I/R that were pretreated with different doses of saffron extract (5 mg/kg, 10 mg/kg, or 20 mg/kg) intraperitoneally. The I/R-induced renal inflammation was assessed by measuring leukocyte infiltration and mRNA expression levels of intercellular adhesion molecule-1 and tumour necrotic factor-alpha. For the assessment of oxidative stress, thiobarbituric acid reactive species and antioxidant capacity of kidneys were measured in the right kidneys. In addition, plasma creatinine and urea-nitrogen concentrations were determined for renal functional disturbances. Statistical analysis was performed using one-way analysis of variance using the Duncan post hoc test. The I/R increased all of the measured parameters, except for the tissue level of ferric reducing/antioxidant power, which decreased. Pretreatment with saffron extract in all doses significantly reduced the severity of these disturbances in such a way that there were no significant differences between the FRAP level and urea-nitrogen concentrations between the sham and all three saffron extract-treated groups. However, the saffron extract could decrease the plasma creatinine concentration, malondialdehyde level, tumour necrotic factor-alpha and intercellular adhesion molecule-1 expression and leukocyte infiltration in a dose-dependent manner. The present study showed anti-inflammatory, anti-oxidative and protective effects for the hydro-ethanolic extract of saffron in I/R-induced acute kidney injury.