اثرات مکمل زعفران بر رابطه بین پروتئین‌های استرس و سایتوکاین‌ها پس از یک جلسه فعالیت جسمانی سنگین در کوهنوردان مرد

هدف این مطالعه بررسی رابطه بین پروتئین شوک حرارتی ۷۰ (HSP70)، اینترلوکین-۶ (IL-6) و فاکتور نکروز تومور-α (TNFα) در فعالیت بدنی شدید با و بدون مکمل زعفران در کوهنوردان مرد بود. ۱۱ نفر شرکت کردند و فعالیت شامل دویدن روی تردمیل با شدت ۸۰-۹۰٪ ضربان قلب بود. نمونه‌های خون پیش، بلافاصله و یک ساعت پس از فعالیت گرفته شد. پس از ۳ روز استراحت، شرکت‌کنندگان روزانه ۳۰۰ میلی‌گرم کپسول زعفران مصرف کردند و فعالیت مجدداً انجام شد. تحلیل‌ها نشان داد TNFα ارتباط منفی قابل توجهی با IL-6 داشت و پس از مکمل، ارتباط مثبتی با HSP70 نشان داد. مکمل زعفران تأثیر مثبت بر پاسخ‌های ایمنی پس از فعالیت بدنی داشت.

The aim of this study was to evaluate the relation between Heat Shock Protein 70 (HSP70), Interleukin-6 (IL-6) and Tumor Necrosis Factor-α (TNFα) in one bout of strenuous physical activity with and without saffron supplementation in male mountain climbers. Participants were eleven male mountain climbers with 27–37 years of age. The physical activity session included running on a treadmill with 5% gradient for 45 min with 80–90% maximum heart rate.

Blood samples were taken in three different times- before, immediately and one h after the physical activity—in order to measure HSP70, IL-6 and TNFα. After three days of recovery, participants started consuming 300 mg/d capsulated dried saffron stigma for10 days. The physical activity session was repeated and blood samples were again taken before, immediately and one h after it. Pearson’s chi-squared test was used for analysis of results (α = 0.05). TNFα has a considerably negative correlation with IL-6 immediately and one h after physical activity.

TNFα showed a significantly positive correlation with HSP70 after supplementation. TNFα and HSP70 concentrations decreased following physical activity both before and after saffron supplementation and their increase was positively correlated to/with each other. Finally Ten days Saffron supplementation showed positive influences on mentioned immune responses after physical activity. Due to supplement’s antioxidant and anti-inflammatory in gredients, a positive correlation between TNFα and HSP70 happened immediately and one h after physical activity